Microinjector with external pressure source, PV850

Microinjection pump PV850 is designed to simplify intracellular injection and a variety of other microinjection tasks. The PV850 uses carefully regulated air pressure for injecting cells with fluid. Injected volumes range from picoliters to nanoliters. The port supplies positive pressure for high-pressure ejection. The pressure port maintains a low positive "compensation" pressure to the injecting pipette between injection pulses to prevent fluid uptake through capillary action.

Timing, injection pressure and compensation pressure are adjusted independently using the touch screen interface and control knob. Time intervals can range from 2 seconds down to 10 ms or less, depending on the injection pressure setting. The injection pressure interval is triggered by using a foot switch, manually, or a computer-controlled TTL pulse.

The PV850 offers separate regulated compensation (back filling prevention) and ejection pressures with a precision timing circuit that switches from injection pressure to compensation pressure automatically.

The display provides easy visibility of key data settings including Injector Pressure, Compensation Pressure, Injection Time, Pump Injection Mode and Injection Count.

Applications of microinjection pump PV850

• Microinjection of diverse compounds and biomolecules – DNA, RNA, proteins
• Pre- and post-implantation in embryos of various species – mice, rats, monkeys, bovine, pigs, zebrafish, etc.